A novel thin film amperometric urea biosensor based on urease-immobilized on poly(N-glycidylpyrrole-co-pyrrole)
| Autor: | Emre Cevik, Mehmet Şenel, M. Fatih Abasıyanık, İbrahim Bozgeyik |
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| Rok vydání: | 2011 |
| Předmět: |
Urease
biology Chemistry technology industry and agriculture Analytical chemistry General Physics and Astronomy macromolecular substances Amperometry chemistry.chemical_compound Covalent bond Urea biology.protein General Materials Science Thin film Fourier transform infrared spectroscopy Biosensor Nuclear chemistry Pyrrole |
| Zdroj: | Current Applied Physics. 11:1083-1088 |
| ISSN: | 1567-1739 |
| DOI: | 10.1016/j.cap.2011.01.041 |
| Popis: | An amperometric biosensor has been developed for the measurement of urea. To construct the proposed biosensor urease enzyme was immobilized onto poly(N-glycidylpyrrole-co-pyrrole) conducting film by direct covalent attachment. The biosensor surface was characterized by FTIR spectroscopy and atomic force microscopy (AFM). The response studies were carried out as a function of urea concentration with amperometric measurements. The biosensor showed a linear current response to the urea concentration ranging from 0.1 to 0.7 mM. The urea biosensor exhibited a sensitivity of 4.5 μA/mM with a response time of 4 s. The factors influencing on the performance of the resulting biosensor were also studied in detail. |
| Databáze: | OpenAIRE |
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