LightCycler? MRSA Advanced Test for Rapid MRSA Detection in Referral Patients Admitted to Intensive Care Units
| Autor: | A. Kongkittimakul, P. Tanyasittisuntorn, Sonchai Hiranniramol, A. Suwanarit, K. Saiam, C. Shinawong, S. Wittayachanyapong, K. Thupvong, C. Vinitwatanakhun, Paithoon Boonma, S. Saenghirunvattana, P. Prakardvudhisarn, R. Kukreja |
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| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
Pediatrics Referral MRSA colonization business.industry biochemical phenomena metabolism and nutrition bacterial infections and mycoses medicine.disease_cause Intensive care unit Methicillin-resistant Staphylococcus aureus law.invention Nasal Swab law Internal medicine Concomitant Intensive care medicine In patient business |
| Zdroj: | The Bangkok Medical Journal. :21-25 |
| ISSN: | 2287-0237 2228-9674 |
| DOI: | 10.31524/bkkmedj.2013.09.004 |
| Popis: | OBJECTIVE: We studied the prevalence of Methicillin Resistant Staphylococcus Aureus (MRSA) nasal carrier in patients who were transferred and admitted to the intensive care unit (ICU). MATERIALS AND METHOD: By using LightCycler® MRSA advanced test for the direct detection of MRSA DNA in nasal colonization by polymerase chain reaction (PCR) and comparing to standard MRSA isolated from plate culture media. RESULTS: From December 2010 to May 2011, 100 patients were enrolled. They were referred to be admitted in ICUs of Bangkok Hospital Medical Center (BMC) (87), Samitivej Hospital Sukhumvit (12) and Bangkok Hospital Pattaya (1). Seventeen patients were excluded from the study. Of 83 patients, the LightCycler® MRSA test detected MRSA from an anterior nasal swab in 12 (14.5%) patients while concomitant plate culture grew MRSA in 10 (12.1%) (Kappa = 0.7913, 95% confi dence interval (CI) = 0.594-0.988). CONCLUSION: The LightCycler® MRSA advanced test is a diagnostic test for rapid MRSA detection. The test aids in the detection of hospital infection and in the control of MRSA infections by rapid detection, therefore identifying the appropiate and isolated patient whom has MRSA colonization particularly in high risk patients. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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