P357 Remote ischemic post-conditioning may prevent cardiac remodeling within two years of STEMI by cardioprotective microRNA upregulation
| Autor: | Alexandra Frogoudaki, Dennis V. Cokkinos, Alkistis Kapelouzou, Spyridon Katsanos, D Vlastos, Ioanna Andreadou, Maria Gazouli, Helen Triantafyllidi, Ignatios Ikonomidis, Efstathios K. Iliodromitis, J Thymis, M Varoudi, Agathi-Rosa Vrettou, C Triantafyllou, G Makavos |
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| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
Conditioning (Psychology) Ventricular End-Systolic Volume business.industry Ischemia Diastole General Medicine medicine.disease Downregulation and upregulation Internal medicine microRNA Cardiology End-diastolic volume Medicine Radiology Nuclear Medicine and imaging Cardiology and Cardiovascular Medicine business Reperfusion injury |
| Zdroj: | European Heart Journal - Cardiovascular Imaging. 21 |
| ISSN: | 2047-2412 2047-2404 |
| Popis: | Background Remote ischemic post-conditioning attenuates ischemia-reperfusion injury in patients with STEMI. However, its biochemical mechanisms, including micro-RNA expression, and effects on cardiac remodeling have not been defined. Methods We examined 80 patients with STEMI and 20 healthy controls. All patients had been evaluated by cardiac echocardiography. The conditioning protocol utilised a single ischemic stimulus by brachial cuff inflation of both arms at 200mmHg for 5 minutes, while 20 patients underwent a sham conditioning procedure by way of cuff inflation omission after its placement. Blood samples were taken before and after the conditioning protocol; thus, the expression of microRNA-144,-150,-499 (cardioprotective action), -21, and -208 (remodeling stimuli) was quantified at baseline and after the ischemic conditioning procedure, by polymerase chain reaction. Additionally, cardiac remodeling was evaluated by repeat echocardiography after a 2-year follow-up period, in 40 patients. Results Our conditioning protocol resulted in a statistically significant increase in miR-144,-150, -499, -21, and -208 expression (55.9 vs 7.4/U6sn, p Conclusion Remote ischemic conditioning may prevent adverse myocardial remodelling within 2 years of the index ischemic event, likely by up-regulation of cardio-protective microRNAs expression. |
| Databáze: | OpenAIRE |
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