| Autor: |
Elise K. Phillips, Jordan A. Cannon, Yue Zhou, Kyle S. Bonifer, Todd B. Reynolds |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Microbiology Spectrum. 11 |
| ISSN: |
2165-0497 |
| DOI: |
10.1128/spectrum.03700-22 |
| Popis: |
Bacillus species are widely used to produce proteins and to understand microbial differentiation. Unfortunately, outside a few lab strains, genetic manipulation is difficult and can prevent thorough dissection of useful phenotypes. We developed a protocol that utilizes conjugation (plasmids that initiate their own transfer) to introduce plasmids into a diverse range of Bacillus spp. This will facilitate a deeper study of wild isolates for both industrial and pure research uses. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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