Conditions for quantitative transformation inAcinetobacter calcoaceticus

Autor:	J. A. Cruze, J. T. Singer, W. R. Finnerty
Rok vydání:	1979
Předmět:	education.field_of_study Growth cycle Growth phase Auxotrophy Population Cell Tryptophan General Medicine Biology Applied Microbiology and Biotechnology Microbiology Molecular biology chemistry.chemical_compound medicine.anatomical_structure chemistry medicine education Incubation DNA
Zdroj:	Current Microbiology. 3:129-132
ISSN:	1432-0991 0343-8651
DOI:	10.1007/bf02601853
Popis:	A transformation procedure that yielded high efficiencies was developed forAcinetobacter calcoaceticus. Strain BD413 Ura− trpE was transformed to tryptophan prototrophy using highly purified DNA. Experimental parameters studied were: (i) recipient cell concentration, (ii) DNA concentration, (iii) growth phase of the recipient cell population, (iv) composition of the growth and transforming medium, and (v) time of incubation of recipient cells with donor DNA. Strain BD413 was competent for transformation throught the growth cycle, with highest competence occurring early in the exponential phase of growth. Maximal transformation efficiencies of 0.5% to 0.7% were obtained in media supporting rapid growth. Recipient cell concentrations of 1×106 to 6×106 cells/ml yielded the highest transformation frequencies, regardless of DNA concentration.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::b6ade925537d905df68313063b8bf72b https://doi.org/10.1007/bf02601853 Zobrazit plný text záznamu Full text from SpringerLink