Young's modulus and SEM analysis of leg bones exposed to simulated microgravity by hind limb suspension (HLS)
| Autor: | Nawab Ali, Michael Soulsby, Niravkumar D. Patel, Parimal Chowdhury, Rahul Mehta |
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| Rok vydání: | 2013 |
| Předmět: |
X-ray spectroscopy
Materials science Scanning electron microscope Resolution (electron density) Energy-dispersive X-ray spectroscopy Analytical chemistry Mineralogy Young's modulus law.invention symbols.namesake law symbols Electron microscope Absorption (electromagnetic radiation) Suspension (vehicle) |
| Zdroj: | AIP Conference Proceedings. |
| ISSN: | 0094-243X |
| Popis: | The aim of this study was to determine composition of the leg bone tissue of rats that were exposed to simulated microgravity by Hind-Limb Suspension (HLS) by tail for one week. The leg bones were cross sectioned, cleaned of soft tissues, dried and sputter coated, and then placed horizontally on the stage of a Scanning Electron Microscope (SEM) for analysis. Interaction of a 17.5 keV electron beam, incident from the vertical direction on the sample, generated images using two detectors. X-rays emitted from the sample during electron bombardment were measured with an Energy Dispersive Spectroscopy (EDS) feature of SEM using a liquid-nitrogen cooled Si(Li) detector with a resolution of 144 eV at 5.9 keV (25Mn Kα x-ray). Kα- x-rays from carbon, oxygen, phosphorus and calcium formed the major peaks in the spectrum. Relative percentages of these elements were determined using a software that could also correct for ZAF factors namely Z(atomic number), A(X-ray absorption) and F(characteristic fluorescence). The ... |
| Databáze: | OpenAIRE |
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