Segmentation of Heterochromatin Foci Using a 3D Spherical Harmonics Intensity Model

Autor:	Andreas Biesdorf, Stefan Wörz, Katharina Müller-Ott, Karl Rohr, Karsten Rippe, Simon Eck
Rok vydání:	2013
Předmět:	Physics Optimization problem Heterochromatin Visual comparison Spherical harmonics Segmentation Algorithm Intensity (heat transfer) Image (mathematics) Parametric statistics
Zdroj:	Bildverarbeitung für die Medizin 2013 ISBN: 9783642364792 Bildverarbeitung für die Medizin
Popis:	We introduce a 3D model-based approach for automatic segmentation of 3D fluorescent heterochromatin foci from 3D microscopy images. The approach employs a new 3D parametric intensity model based on a spherical harmonics (SH) expansion and can represent foci of regular and highly irregular shapes. By solving a least-squares optimization problem, the model is directly fitted to the 3D image data, and the model parameters including the SH expansion coefficients are estimated. The approach has been successfully applied to real 3D microscopy image data. A visual comparison and a quantitative evaluation show that the new approach yields better results than previous approaches.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::b699065bed1a7dee5b71e9fb3a8ee291 https://doi.org/10.1007/978-3-642-36480-8_54 Zobrazit plný text záznamu