Lack of neuropeptide Y receptor detection in human bladder and prostate

Autor: B. J. Davis, Russ Chess-Williams, S. Bein, Mark Goepel, Martin C. Michel, Christopher R. Chapple
Rok vydání: 2001
Předmět:
Zdroj: BJU International. 85:918-924
ISSN: 1464-410X
1464-4096
Popis: Objective To investigate the presence of functional neuropeptide Y (NPY) receptors in human bladder and prostate (both richly endowed with NPY-containing nerve fibers) using peptide YY (PYY) as the agonist. Materials and methods Binding studies were conducted using [125I]PYY as the radioligand. Organ-bath studies were performed on isolated tissue strips for direct (postjunctional) contractile effects and for (prejunctional) inhibition of field stimulation effects. Any possible degradation of PYY was determined using high-performance liquid chromatography (HPLC). Results In the radioligand binding studies no quantifiable specific [125I]PYY binding was detected in human bladder or prostate, while specific high-affinity binding was readily seen in rat cerebral cortex. In organ-bath experiments, PYY (up to 1 µmol/L) caused no contraction of human prostate or bladder, whereas noradrenaline and carbachol, respectively, were effective; the potency or efficacy of noradrenaline and carbachol were not altered by PYY. Field stimulation-induced contraction was not affected by PYY in either human bladder or prostate, but was readily inhibited in rat vas deferens. HPLC detected no relevant PYY degradation by human bladder or prostate homogenates. Conclusion Human bladder and prostate express only very few if any functional NPY receptors.
Databáze: OpenAIRE
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