| Autor: |
M.K. Khubetsova, S.A. Borzenok, Dentistry, Moscow, Russian Federation, А.А. Zheltonozhko, S.V. Kostenev, V.G. Li, D.S. Ostrovskiy |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Modern technologies in ophtalmology. :220-223 |
| ISSN: |
2312-4911 |
| Popis: |
Purpose. Conduct a comparative analysis of corneal tissue–engineered constructs that have undergone cryopreservation of three different cryoprotectants. Materials and methods. After refractive surgery Relex SMILE, corneal tissue was obtained which is called a lenticule. Corneal tissue engineering constructs (RTK) have created using decellularization methods that relate to the technologies of tissue and regenerative medicine. For decellularization of the lenticules, a protocol with a 1.5 M solution of Sodium Chloride with DNase 5 U / ml and RNase 5 U / ml was used. After decellularization, RTK was cryopreserved using: 1) 10% DMSO and 90% “corneal storage solution” (Borzenok–Moroz medium), 2) «Cryoderm» cell cryopreservation medium, 3) 100% glycerin. Transparency was assessed using spectrophotometry. The thickness of collagen fibers was assessed by scanning electron microscopy (SEM). Results. When assessing the transparency of the control and experimental groups, a statistically significant decrease in transparency was revealed in the groups using cryoprotectants – Glycerin and Cryoderm (p0.05). The Glycerol–Control group was statistically different (p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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