Modulation of NAD+ biosynthesis activates SIRT1 and resists cisplatin-induced ototoxicity
Autor: | Yiqing Zheng, Yongyi Ye, Haidi Yang, Ting Zhan, Wuhui He, Xin Min, Xiaotong Huang, Jiaqi Pang, Hao Xiong, Weijian Zhang, Feinan He, Zhengrong Liang, Bingquan Jian, Yiming Gao |
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Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Cisplatin biology AMPK General Medicine Nicotinamide adenine dinucleotide Pharmacology Toxicology medicine.disease 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine PARP1 medicine.anatomical_structure chemistry Ototoxicity Sirtuin medicine biology.protein NAD+ kinase Hair cell 030217 neurology & neurosurgery medicine.drug |
Zdroj: | Toxicology Letters. 349:115-123 |
ISSN: | 0378-4274 |
Popis: | Cisplatin, the most widely used platinum-based anticancer drug, often causes progressive and irreversible sensorineural hearing loss in cancer patients. However, the precise mechanism underlying cisplatin-associated ototoxicity is still unclear. Nicotinamide adenine dinucleotide (NAD+), a co-substrate for the sirtuin family and PARPs, has emerged as a potent therapeutic molecular target in various diseases. In our investigates, we observed that NAD+ level was changed in the cochlear explants of mice treated with cisplatin. Supplementation of a specific inhibitor (TES-1025) of α-amino-β-carboxymuconate-e-semialdehyde decarboxylase (ACMSD), a rate-limiting enzyme of NAD+de novo synthesis pathway, promoted SIRT1 activity, increased mtDNA contents and enhanced AMPK expression, thus significantly reducing hair cells loss and deformation. The protection was blocked by EX527, a specific SIRT1 inhibitor. Meanwhile, the use of NMN, a precursor of NAD+ salvage synthesis pathway, had shown beneficial effect on hair cell under cisplatin administration, effectively suppressing PARP1. In vivo experiments confirmed the hair cell protection of NAD+ modulators in cisplatin treated mice and zebrafish. In conclusion, we demonstrated that modulation of NAD+ biosynthesis via the de novo synthesis pathway and the salvage synthesis pathway could both prevent ototoxicity of cisplatin. These results suggested that direct modulation of cellular NAD+ levels could be a promising therapeutic approach for protection of hearing from cisplatin-induced ototoxicity. |
Databáze: | OpenAIRE |
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