Two Different Banana NPR1-Like Coding Sequences Confer Similar Protection Against Pathogens in Arabidopsis

Autor: Rosita E. Yocgo, Gary Creissen, Rachel Chikwamba, Karl J. Kunert
Rok vydání: 2012
Předmět:
Zdroj: Tropical Plant Biology. 5:309-316
ISSN: 1935-9764
1935-9756
Popis: Differences between the coding sequences of two banana homologues of the Non-expressor of Pathogenesis-Related 1 (NPR1) genes (MNPR1A and MNPR1B) were investigated as a possible cause for the differential activity of the two genes. Each of the MNPR1 coding sequences were expressed under the control of the cauliflower mosaic virus 35S promoter/terminator sequences in the transgenic Arabidopsis npr1-2 mutant. These MNPR1-expressing plants were then exposed to either the biotrophic oomycete Hyaloperonospora arabidopsidis, the necrotrophic fungus Botrytis cinerea, or the hemi-biotrophic bacterial pathogen Pseudomonas syringae. Expression of either MNPR1A or MNPR1B increased Pathogenesis-related 1 (PR-1) transcription in a similar manner in mutant plants and reduced pathogen growth, restoring resistance of the Arabidopsis npr1-2 mutant plants to pathogens. Sequence differences between MNPR1A and MNPR1B coding sequences did not affect MNPR1activity, suggesting a possible role for the involvement of other regulatory sequences in differential MNPR1 gene expression.
Databáze: OpenAIRE
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