Time-resolved resonance Raman studies of bacteriorhodopsin and its intermediates K590 and L550: Biological implications

Autor: M. Stockburger, R. Lohrmann
Rok vydání: 1992
Předmět:
Zdroj: Journal of Raman Spectroscopy. 23:575-583
ISSN: 0377-0486
Popis: Experiments with a spinning cell and a single continuous-wave laser beam (514 nm) in reflection geometry were performed to obtain the resonance Raman (RR) spectra of the intermediate K 590 of the retinal chromophore of bacteriorhodopsin (bR). Samples of diluted aqueous suspensions of «purple membranes» from Halobacterium halobium were used, which entail bR as an integral protein. K 590 is formed photochemically in ca. 5 ps from the parent chromophore BR 570 and in H 2 O decays into the product L 550 with a time constant of ca. 1.2 μs (21°C)
Databáze: OpenAIRE