Quantification of ZP1, ZP2 and ZP3 mRNA of marmoset monkey (Callithrix jacchus) oocytes from periantral and antral follicles
| Autor: | Elvira Hinsch, P. Nayudu, S. Kuhnert, R. Einspanier, Lutz Konrad, K.-D. Hinsch |
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| Rok vydání: | 2011 |
| Předmět: |
endocrine system
medicine.medical_specialty biology Urology Acrosome reaction Marmoset Embryo General Medicine Antral follicle biology.organism_classification Oocyte Callithrix Andrology Endocrinology medicine.anatomical_structure Internal medicine biology.animal medicine Folliculogenesis Zona pellucida |
| Zdroj: | Andrologia. 44:349-353 |
| ISSN: | 0303-4569 |
| DOI: | 10.1111/j.1439-0272.2011.01188.x |
| Popis: | Summary In mammals, the oocyte and preimplantation embryo are protected by the zona pellucida (ZP) consisting mainly of ZP glycoproteins, which are responsible for sperm binding, induction of the acrosome reaction and zona pellucida hardening to prevent polyspermia. The ZP proteins become increasingly important as possible predictors for in vitro cultured oocytes competence. As little is known about the stage-dependent expression of ZP1, ZP2 and ZP3 in marmoset monkey (Callithrix jacchus) oocytes, mRNA expression was investigated with realtime RT-PCR. Total-RNA was isolated from three different classes of marmoset oocytes; Class 1 oocytes from periantral follicles ( 1000 lm, n = 9). Compared with Class 1 oocytes mRNA expression of ZP1, ZP2 and ZP3 in Class 2 oocytes was significantly decreased. In Class 3 oocytes, the transcription of ZP1, ZP2 and ZP3 genes showed also a significant decrease compared with Class 1 oocytes. In this study a differently regulated expression of the ZP genes during late folliculogenesis with an obvious downregulation of ZP1, ZP2 and ZP3 could be demonstrated for the first time in the marmoset monkey. |
| Databáze: | OpenAIRE |
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