Sulphate ion influx and efflux inhydrodictyon reticulatum

Autor:	Renata Rybová, Ludmila Nešpůrková, Karel Janáček
Rok vydání:	1988
Předmět:	Absorption (pharmacology) Chromatography Substrate (chemistry) Plant Science Horticulture Membrane transport Phosphate chemistry.chemical_compound chemistry DIDS Efflux Sulfate Ion transporter Nuclear chemistry
Zdroj:	Biologia Plantarum. 30:440-450
ISSN:	1573-8264 0006-3134
DOI:	10.1007/bf02890514
Popis:	Sulphate ion is accumulated in the cells of the algaHydrodidyon reticulatum to a concentration approximately 30 mmol 1-1. The rate of SO42- uptake is increased markedly in the light, however, the effect is observable only after the cells have been kept in the light for about one hour. Uptake of sulphate is strongly inhibited by phosphorylation uncouplers and also by DIDS. The two-phase sulphate uptake kinetics, reported earlier was confirmed in experiments in which competition with phosphate anion was tested. Phosphate competes with sulphate only in the range of higher substrate concentrations (from 0.2 mmol 1-1) and does not affect the system which works at low substrate concentrations. Efflux of SO42- is very slow; the reason of the flux hindrance is not yet known.
Databáze:	OpenAIRE
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