HVEM of Central Nervous System Tissue: Localization of the Site of Physiologic Measures
| Autor: | R. J. Brady, J. N. Turner, J. W. Swann, D. N. Collins, D. O. Carpenter |
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| Rok vydání: | 1985 |
| Předmět: | |
| Zdroj: | Proceedings, annual meeting, Electron Microscopy Society of America. 43:586-587 |
| ISSN: | 2690-1315 0424-8201 |
| DOI: | 10.1017/s0424820100119715 |
| Popis: | The correlation of physiology and ultrastructure is critical to many areas of neurobiology, but is usually possible only in “simple” systems, (neuromuscular junction, isolated invertebrate or lower vertebrate preparation or in culture). Such correlation is difficult in the central nervous system (CNS) due to its complex microanatomy, large number of synapses on each neuron and lack of techniques for finding specific sites, (e.g. presynaptic terminals buried in a mass of tissue). We are developing specimen labeling and preparation methods to mark the site at which a physiologic parameter has been measured or manipulated by a microelectrode. The marker must be either electron dense or fluorescent - the former is preferred. It must bind to the cell surface without excessive diffusion into surrounding tissue, and it must be visible during block trimming and sectioning, to form a traceable link from the macroscopic to the ultrastructure. |
| Databáze: | OpenAIRE |
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