On-Chip Experiment System for Evaluation of Local Reaction of a Single Cell
| Autor: | Hisataka Maruyama, Toshio Fukuda, Fumihito Arai, Tohoru Katsuragi |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Materials science
Microchannel business.industry Mechanical Engineering technology industry and agriculture Nanotechnology Chip Laser Fluorescence Industrial and Manufacturing Engineering law.invention Mercury-vapor lamp Fixation (surgical) Optical tweezers Mechanics of Materials law Optoelectronics Irradiation business |
| Zdroj: | TRANSACTIONS OF THE JAPAN SOCIETY OF MECHANICAL ENGINEERS Series C. 70:3550-3555 |
| ISSN: | 1884-8354 0387-5024 |
| Popis: | In recent years, single cell experiment has become very important for investigating unknown cell properties. We developed a novel technique to investigate individual cell properties on a chip using newly developed cell fixation method by the photo-crosslinkable resin. We propose three different fixation methods, that is, direct fixation method, caging method, and direct fixation with position control method. The isolated cell is fixed in the cured resin directly or inside the cage of the cured resin. The laser tweezers is employed to manipulate the target cell indirectly by using the laser trapped droplet of the resin as a microtool. The cell is positioned properly by the laser tweezers and is fixed in the cured resin. We tested the mercury lamp as well as the He Cd laser for UV ray irradiation at the local area. The fixation force of the resin is strong enough to stay with the drag force by the microchannel flow at the speed more than 466 mm/s. In experiment, we confirmed the single cell fixation by the proposed fixation methods. Then, we succeeded in a series of reaction experiments, such as culture and fluorescent reaction on a chip. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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