The heat shock-induced cell cycle arrest is attenuated by weak electromagnetic fields
| Autor: | Sergey V. Tokalov, Herwig O. Gutzeit |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
education.field_of_study
Cell cycle checkpoint medicine.diagnostic_test Population Cell Carboxyfluorescein succinimidyl ester Cell Biology General Medicine Cell cycle Biology Flow cytometry chemistry.chemical_compound medicine.anatomical_structure chemistry Apoptosis Shock (circulatory) medicine Biophysics medicine.symptom education |
| Zdroj: | Cell Proliferation. 36:101-111 |
| ISSN: | 0960-7722 |
| DOI: | 10.1046/j.1365-2184.2003.00261.x |
| Popis: | Stress-induced effects in human acute leukaemia cells (HL-60) were studied by flow cytometry using the fluorescent dye carboxyfluorescein succinimidyl ester which allows the analysis of several successive cell generations for up to 10 days. Asynchronously cycling cells subjected to heat shock (30 min at 41 degrees C) responded in two distinct ways: while one fraction of the cell population (about 15%) re-entered the cell cycle after a short delay, other cells became arrested at different phases of the cell cycle and remained arrested for up to several days and finally underwent apoptosis. Weak electromagnetic fields (60 micro T, 50 Hz) alleviated the heat-induced block and the fraction of arrested cells was significantly smaller. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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