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OBJECTIVE The aim: To determine the morphometric parameters of the parenchymal and stromal liver components of healthy newborns. PATIENTS AND METHODS Material and methods: The morphometric investigation included 45 liver tissue biopsies of healthy newborns. All morphometric parameters of the parenchymal and stromal liver components were calculated using the Avtandilov microscopic morphometric grid. It was inserted into the microscope ocular tube with a total × 200 microscope magnification. The number of points that were found on the corresponding types of parenchymal and stromal liver components was calculated. In every case, it was selected 10 random microscopic areas and then all data were obtained, calculated and presented as percentages. RESULTS Results: Morphometric parameters of hepatocytes: mononuclear hepatocytes - 93.5±7.1 %, two-nuclear hepatocytes - 6.5±1.2 %, BMHC (bi-/mononuclear hepatocytes coefficient) - 0.06±0.01, hepatocytes with fat vacuoles - 0.5±0.2 %. Parenchymal and stromal liver components: parenchyma - 74.2±4.3 %, stroma (including blood vessels and bile ducts) - 25.8±2.6 %, SPI (stroma/parenchyma index) - 0.34±0.01. Morphometric parameters of all of the liver components: hepatocytes - 74.2±4.3 %, portal tracts - 3.1±0.6 %, central veins - 9.3±1.4 %, sinusoids - 10.5±1.3 %, bile ducts - 2.9±0.2 %. Expression level parameters: fibronectin - 17.3±2.5 %, collagen type I - 9.7±1.9 %, collagen type III - 10.1±0.9 %, collagen type IV - 5.9±0.2 %. Parameters of liver fibrosis biomarkers: APRI (index) - 0.19±0.01, а FIB-4 (index) - 0.022±0.001. CONCLUSION Conclusions: The morphometric parameters of the parenchymal and stromal liver components of healthy newborns can be used as a control group in the study of any pathological conditions of the liver of newborns. |
