High Quality Independent From a Donor: Human Amniotic Fluid Derived Stem Cells-A Practical Analysis Based on 165 Clinical Cases
| Autor: | Łukasz Kaźmierski, Dorota Porowińska, Pawel Walentowicz, Anna Bajek, Andrzej Marszałek, Pawel Sadlecki, Robert Dębski, Magdalena Bodnar, Joanna Olkowska, Marek Grabiec, Joanna Czarnecka, Tomasz Drewa, Malgorzata Walentowicz-Sadlecka |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Amniotic fluid medicine.diagnostic_test Cell Mesenchymal stem cell Adipose tissue Karyotype Cell Biology Biology Biochemistry Andrology 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure medicine Amniocentesis Stem cell Induced pluripotent stem cell Molecular Biology |
| Zdroj: | Journal of Cellular Biochemistry. 118:116-126 |
| ISSN: | 0730-2312 |
| DOI: | 10.1002/jcb.25618 |
| Popis: | The aim of the study was to extend the potential use of human stem cells isolated from amniotic fluid in medical applications by confirming their high homogeneity and quality. Amniotic fluid samples were collected during amniocentesis from 165 women during pregnancy. The proliferation rate, clonogenicity, karyotype, aging process, pluripotent cell markers, expression of surface markers, and the potential to differentiate into adipose, bone and cartilage cells of hAFSCs were analyzed. Obtained results revealed that mesenchymal stem cells could be derived successfully from amniotic fluid, which exhibit properties comparable with MSCs of other origins. It is the first study, in which such a large group of patients was involved. Comprehensive statistical and biological analysis were conducted some of which clearly being innovative in relation to human amniotic fluid-derived stem cells. J. Cell. Biochem. 118: 116-126, 2017. © 2016 Wiley Periodicals, Inc. |
| Databáze: | OpenAIRE |
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