Detection of GB Virus-C/Hepatitis G Virus RNA in Serum by Reverse Transcription Polymerase Chain Reaction

Autor:	Jungsuh P. Kim, Mickey S. Urdea, Janice A. Kolberg, George G. Schlauder, Bhavna Bhardwaj, Masashi Mizokami, Gary L. Davis, Ke-Ping Qian, Johnson Y.N. Lau, Jeffrey M. Linnen, Jill Detmer
Rok vydání:	1997
Předmět:	NS3 biology viruses virus diseases RNA Amplicon biology.organism_classification Virology Molecular biology GB virus C digestive system diseases Virus Hepatitis B virus PRE beta Reverse transcription polymerase chain reaction Flaviviridae Infectious Diseases
Zdroj:	Journal of Medical Virology. 52:92-96
ISSN:	1096-9071 0146-6615
DOI:	10.1002/(sici)1096-9071(199705)52:1<92::aid-jmv15>3.0.co;2-2
Popis:	Three PCR methods based on the GB virus-C/hepatitis G virus (GBV-C/HGV) 5′UTR and NS3 genomic region were used for the detection of GBV-C/HBV RNA in serum of 62 patients with chronic hepatitis C virus (HCV) infection. Ten of 62 (16%) patients were found to have GBV-C/HGV RNA, which was confirmed by sequence analysis of the 5′UTR PCR amplicon. All methods appear to be specific, but methods based on the 5′UTR appear to be more sensitive. J. Med. Virol. 52:92–96, 1997. © 1997 Wiley-Liss, Inc.
Databáze:	OpenAIRE
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