Molybdenum Speciation in Raw Phloem Sap of Castor Bean

Autor: Fida Madayanti Warganegara, Noor Fitri, Bernd Kastenholz, Buchari, Muhammad Bachri Amran
Rok vydání: 2008
Předmět:
Zdroj: Analytical Letters. 41:1773-1784
ISSN: 1532-236X
0003-2719
Popis: Separation and quantification of molybdenum (Mo) in raw phloem sap from castor bean (Ricinus communis L.) was performed by size exclusion chromatography (SEC) and further purification was performed using quantitative preparative native continuous polyacrylamide gel electrophoresis (QPNC-PAGE). For elemental detection, an inductively coupled plasma quadrupole mass spectrometer (ICP-QMS) was applied. Two different SEC columns were utilized: column A, Sephadex G-50 SF (700 mm × 24 mm), and column B, Sephadex G-25 M (28 mm × 9 mm). The protein content of the fractions was determined by the Bradford method. Using column A, two peaks of Mo were detected consisting of a main peak (MoA2) in the low molecular weight area (
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje