Impaired secretion and fluid-phase endocytosis in the End4 mutant of Chinese hamster ovary cells

Autor:	Elizabeth A. Rutledge, Ru Hung Wang, Rockford K. Draper, Penelope A. Colbaugh, Chia Yi Kao
Rok vydání:	1990
Předmět:	chemistry.chemical_classification Chinese hamster ovary cell Endocytic cycle Hamster Cell Biology Receptor-mediated endocytosis Biology Endocytosis Biochemistry Molecular biology Cell biology chemistry Transferrin Secretion Molecular Biology Secretory pathway
Zdroj:	Journal of Biological Chemistry. 265:20179-20187
ISSN:	0021-9258 2211-2219
DOI:	10.1016/s0021-9258(17)30487-8
Popis:	To see what effect the lesion had on the endocytic pathway, we measured the accumulation and recycling of the fluid-phase marker horseradish peroxidase. Accumulation was inhibited by 50% while recycling was barely affected, suggesting that the rate of fluid-phase endocytosis was reduced. We previously showed that the clathrin-coated pit pathway of endocytosis was not affected in the mutant, indicated by a normal transferrin cycle (Colbaugh, P. A., Stookey, M., and Draper, R. K. (1989) J. Cell Biol. 108,2211-2219). Thus, the secretory lesion correlates with reduced fluid-phase endocytosis without impairing the clathrin-dependent pathway of receptor-mediated endocytosis. We also investigated the delivery of endocytosed material to lysosomes and found that delivery was partially, but not completely, impaired in the mutant. This suggests that endocytosed material can enter lysosomes, although slowly, in the absence of a functional secretory pathway.
Databáze:	OpenAIRE
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