Finding of regulatory codes in 5'-UTR of A. thaliana mRNAs by polysome profiling method

Autor: K. V. Kabardaeva, I. V. Deineko, I. V. Goldenkova-Pavlova, O. N. Mustafaev, Aleksandra V. Suhorukova
Rok vydání: 2020
Předmět:
Zdroj: Abstract book of the 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology" PLAMIC2020.
Popis: The polysome profiling method was used to separate mRNAs depending on their loading by ribosomes into polysomal and monosomal fractions. Pools separation of such mRNAs and analysis of transcripts (mRNAs) which are associated with each mRNA pool due to RNA sequencing allowed to get an idea of the translational efficiency of individual mRNAs. Moreover, subsequent in silico analysis make possible searching of regulatory contexts in the 5'-UTR of plant A. thaliana, which may be potentially important for efficient translation of mRNA.
Databáze: OpenAIRE