Dipeptidase-Inactivated tACE Action In Vivo: Selective Inhibition of Sperm-Zona Pellucida Binding in the Mouse1
| Autor: | Eishi Deguchi, Hitomi Watanabe, Gen Kondoh, Taeko Tani, Shuichi Yamada |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Dipeptidase
Genetically modified mouse medicine.medical_specialty biology urogenital system Angiotensin-converting enzyme Cell Biology General Medicine Epididymis Sperm Cell biology medicine.anatomical_structure Human fertilization Endocrinology Reproductive Medicine Internal medicine Knockout mouse medicine biology.protein Zona pellucida |
| Zdroj: | Biology of Reproduction. 77:794-802 |
| ISSN: | 1529-7268 0006-3363 |
| Popis: | The angiotensin-converting enzyme (ACE) plays a crucial role in male fertilization and is a key regulator of blood pressure. Testicular ACE (tACE), the germinal specific isozyme expressed on different promoters, exclusively carries out the role of ACE in fertility, although the site and mode of action are not well known. To investigate the contribution of tACE in fertilization, we produced transgenic mouse lines carrying a dipeptidase-inactivated mutant. Although the transgenic mice showed normal blood pressure, kidney morphology, and fertility, reduced fertilization was observed after in vitro fertilization (IVF). The sperm-zona pellucida (ZP) binding was exclusively impaired in these lines in a manner similar to that observed in an Ace knockout mouse. The dipeptidase activity was reduced in epididymal ingredients but not in the testis. Furthermore, direct application of mutant protein did not suppress sperm-ZP binding of intact sperm during IVF, implying that the dipeptidase-inactivated mutant affects sperm modification in the epididymis for ZP binding. Our results indicate that the dipeptidase-inactivated tACE acts in vivo, suggesting that tACE contributes to fertilization as a dipeptidase at least in the epididymis. |
| Databáze: | OpenAIRE |
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