Response of Pisum Sativum Cytokinin Oxidase/Dehydrogenase Expression and Specific Activity to Drought Stress and Herbicide Treatments

Autor:	Irina Vaseva-Gemisheva, Emanuil Karanov, David Lee
Rok vydání:	2005
Předmět:	Physiology fungi food and beverages Plant physiology Dehydrogenase Plant Science Biology biology.organism_classification Medicago truncatula Pisum chemistry.chemical_compound Sativum Biochemistry Glufosinate chemistry Gene expression Botany Cytokinin Agronomy and Crop Science
Zdroj:	Plant Growth Regulation. 46:199-208
ISSN:	1573-5087 0167-6903
DOI:	10.1007/s10725-005-0143-3
Popis:	The expression of cytokinin oxidase/dehydrogenase (CKX EC: 1.5.99.12) is subject to fine regulation and it provides a rapid turnover of cytokinins, which serves as a signal for triggering developmental events during plant growth. The activity of this enzyme is believed to be responsible for the changes in cytokinin pool under adverse environmental conditions. CKX gene-specific assay to measure the expression in response to different stress treatments in the tissues of Pisum sativum plants was developed. Pea CKX genes were amplified and sequenced using primers designed from the sequences of Medicago truncatula CKX genes. Expression of two P. sativum CKX genes was assessed using relative-quantification in real time two-step RT-PCR, in leaves and roots of drought-, glufosinate- and atrazine-treated cv. Manuela pea plants. Varied CKX responses support the existence of complicated regulating mechanism of cytokinin oxidase/ dehydrogenase gene expression.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::b5e82504a71046c6f2482843bc5e161a https://doi.org/10.1007/s10725-005-0143-3 Zobrazit plný text záznamu Full text from SpringerLink