| Autor: |
Mir Hadi Jazayeri, Farideh Siavoshi, Parastoo Saniee, Abdolfattah Sarrafnejad, Hoda Ebrahimi, Maryam Mobini |
| Rok vydání: |
2021 |
| Předmět: |
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| Popis: |
Observing release of large extracellular vesicles (EVs) from yeast cell raised the question about size and flexibility of cell wall pores. To estimate the approximate size of pores, internalization of nanoparticles (NPs) with different sizes, into the intact yeast cell was examined. Candida tropicalis was cultured in N-acetylglucoseamine-yeast extract broth (NYB) and examined every 12 hr by light microscopy for observing release of EVs. Yeast culture in NYB was treated with Fluorescein isothiocyanate (FITC) -labelled NPs; gold (45, 70 and 100 nm), albumin (100 nm) and Fluospheres (1000 and 2000 nm). Fluorescence microscope was used for recording internalization of NPs with different concentrations (0.1-10%) after few seconds to 120 min of treatment. Release of EVs with different size and morphology from yeast mostly occurred at 36 hr. Best NPs’ concentration was 0.1% and internalization occurred within few seconds after treatment. Positively charged 45 nm gold NPs internalized into yeast cell and accumulated in the vacuole and 100 nm gold NPs destroyed the yeast cell. Gold NPs with 70 nm size and 100 nm negatively charged albumin NPs were internalized into the vacuole of a few yeast cells. Inert Fluospheres were first degraded outside the yeast’s cell and then absorbed into yeast’s cell and finally reached the vacuole. Release of large EVs from yeast indicated flexibility of cell wall pores and EVs for remodelling. Furthermore, physicochemical properties of internalizing NPs and those of cell wall determined uptake of NPs by yeast cell. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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