Reduced Expression of Stromal-Derived Factor 1 in Autonomous Thyroid Adenomas and Its Regulation in Thyroid-Derived Cells1
| Autor: | Gabriela Aust, M. Kamprad, M. Steinert, C. Simchen, S. Kiessling |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
medicine.medical_specialty
Chemokine endocrine system diseases biology Adenoma Endocrinology Diabetes and Metabolism Graves' disease Biochemistry (medical) Clinical Biochemistry Thyroid adenoma Thyroid medicine.disease Biochemistry Chemokine receptor Endocrinology medicine.anatomical_structure Thyroid peroxidase Internal medicine biology.protein medicine Stromal cell-derived factor 1 |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 86:3368-3376 |
| ISSN: | 1945-7197 0021-972X |
| DOI: | 10.1210/jcem.86.7.7654 |
| Popis: | Stromal-derived factor 1 (SDF-1) and CXCR4 comprise a unique chemokine/chemokine receptor pair, exhibiting important functions in morphogenesis and growth regulation as well as attractant properties on T lymphocytes. No data are available on SDF-1 and CXCR4 in normal or pathological thyroid tissues. SDF-1, CXCR4, and CD18 messenger ribonucleic acid (mRNA) as a marker of leukocytic infiltration were quantified in tissues affected by thyroid adenoma (n = 11) and Graves’ disease (GD; n = 16) using competitive RT-PCR. SDF-1 mRNA levels differed significantly between autonomous adenomas and the corresponding normal tissue, but not in GD between patients with low or high leukocyte infiltration, thyroid peroxidase, and TSH receptor autoantibodies, respectively. We found a strong correlation between CXCR4 and CD18 mRNA, which indicates CXCR4 expression by leukocytes. To define the cellular source of SDF-1 and CXCR4 in thyroid tissue, we examined various thyroid-derived cells. Fibroblasts are the most potent produ... |
| Databáze: | OpenAIRE |
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