Invitro and invivo cultivation of Pergularia tomentosa for cardenolides

Autor:	Youssef A.K, M.A. El-Shanawany, Khalifa M.M., Hifnawy M.S., M.H Bekhit, S.Y Desoukey
Rok vydání:	2014
Předmět:	chemistry.chemical_classification Pergularia tomentosa Hplc analysis fungi Ghalakinoside Glycoside Biology In vitro Tissue culture Horticulture chemistry In vivo Botany Explant culture
Zdroj:	IOSR Journal of Pharmacy and Biological Sciences. 9:40-52
ISSN:	2278-3008 2319-7676
DOI:	10.9790/3008-09214052
Popis:	The importance for the conservation of the Egyptian desert plant Pergularia tomentosa was encouraged after the isolation and identification of the highly stable and water soluble cardiac glycosides with very interesting pharmacological activities and wide safety margins. The study included agricultural and tissue culture experiments for the possible production of these important cardenolides in high concentrations. Calli formation and growth obtained from different explants were significantly affected by many factors tested. The HPLC analysis of the extracts of both cultivated plants samples and calli from tissue culture experiments demonstrated promising results. All extracts showed the presence of the major ghalakinoside, in variable concentrations. The highest results were obtained from the irrigated plants in the agricultural experiments and after progesterone addition in tissue culture experiments.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::b5b05b5200718e9a4898509a66ffc702 https://doi.org/10.9790/3008-09214052 Zobrazit plný text záznamu