Biochemical reactions of Brevibacterium flavum depending on medium stirring intensity and flow structure

Autor: U. E. Viesturs, M. P. Ruklisha, M. K. Toma, M. A. Rikmanis, J. J. Vanags
Rok vydání: 1989
Předmět:
Zdroj: Acta Biotechnologica. 9:565-577
ISSN: 1521-3846
0138-4988
Popis: Authors have studied the dependence of the physiological and biochemical characteristics of lysine producers Brevibacterium flavum on medium stirring intensity upon constant pO2 during fermentation processes. Various factors affecting the rate of biochemical reactions in cells upon a changeable intensity of medium stirring in apparata with turbine stirrers; oxygen mass transfer intensity in the system; stirring characteristics in the fermenter; biochemical changes in the cells due to gradients of energy introduced by the stirrer in the fermenter, etc. have been studied. A comparison of the experimentally established values, characterizing macrostirring (e) and microstirring (KR), with the physiological and chemical characteristics of bacteria during fermentation made us suppose that the character of the kinetic energy distribution in the volume of fermenter is one of the factors regulating bacterial metabolism. Besides, there exists the value ecrit, upon which there can be observed essential changes of the physiological and biochemical properties of the cells. The effect of medium flows upon the cells is characterized by index Fstress, which takes into account both, the possible presence of the cells in the zone with e ≧ ecrit, and the degree of medium flow and cell interaction, numerically equal to value (e – ecrit)0.5.
Databáze: OpenAIRE