Hepatocytes in Primary Culture: An Alternative to LD50 Testing? Validation of a Predictive Model by Multivariate Analysis
| Autor: | Jean-Paul Cano, Nicole Bichet, Anne-Françoise Peloux, Daniel Gouy, Christian Fédérici |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
medicine.medical_specialty
Neutral red General Medicine Biology Toxicology General Biochemistry Genetics and Molecular Biology In vitro Surgery Andrology Medical Laboratory Technology chemistry.chemical_compound medicine.anatomical_structure chemistry Cell culture Lactate dehydrogenase Hepatocyte Toxicity medicine MTT assay Cytotoxicity |
| Zdroj: | Alternatives to Laboratory Animals. 20:8-26 |
| ISSN: | 2632-3559 0261-1929 |
| DOI: | 10.1177/026119299202000103 |
| Popis: | The cytotoxicity of 30 chemicals was assessed in rat hepatocyte primary cultures using four methods: lactate dehydrogenase release, neutral red uptake, the MTT assay, and measurement of total protein content. Comparison of the data obtained in vitro (IC50 values) and in vivo (LD50 values) resulted in a significant correlation (p Assessment of the cytotoxicity of 54 additional chemicals would provide a more accurate predictability limit around l,500μg/ml and the estimated predictability confidence interval could be reduced to 90–100%. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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