Development of Lateral Flow Immunoassay for Antigen Detection in Human Angiostrongylus cantonensis Infection
| Autor: | Mu-Xin Chen, Ren-Li Zhang, Jia-Xu Chen, Da-Na Huang, Shao-Hong Chen, Lin Ai |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.drug_class 030231 tropical medicine 030108 mycology & parasitology Biology Monoclonal antibody Angiostrongylus cantonensis infection medicine.disease biology.organism_classification Virology Serology Angiostrongylus cantonensis 03 medical and health sciences Elisa kit 0302 clinical medicine Infectious Diseases Antigen Immunology medicine Angiostrongyliasis Parasitology Lateral flow immunoassay |
| Zdroj: | The Korean Journal of Parasitology. 54:375-380 |
| ISSN: | 1738-0006 0023-4001 |
| DOI: | 10.3347/kjp.2016.54.3.375 |
| Popis: | Angiostrongyliasis is difficult to be diagnosed for the reason that no ideal method can be used. Serologic tests require specific equipment and are not always available in poverty-stricken zone and are time-consuming. A lateral flow immunoassay (LFIA) may be useful for angiostrongyliasis control. We established a LFIA for the diagnosis of angiostrongyliasis based on 2 monoclonal antibodies (mAbs) against antigens of Angiostrongylus cantonensis adults. The sensitivity and specificity were 91.1% and 100% in LFIA, while those of commercial ELISA kit was 97.8% and 86.3%, respectively. Youden index was 0.91 in LFIA and 0.84 in commercial ELISA kit. LFIA showed detection limit of 1 ng/ml of A. cantonensis ES antigens. This LFIA was simple, rapid, highly sensitive and specific, which opened an alternative approach for the diagnosis of human angiostrongyliasis. |
| Databáze: | OpenAIRE |
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