Polymerase chain reaction assay for the detection ofDendrolimus kikuchiinucleopolyhedrovirus
| Autor: | Yu Zhu Wang, Zhi Lin Zhang, Yong An Zhang, Yan Li Duan, Qing Hua Wang, Meng Lou Li, Jian Liang Qu, Xiao Ning Nan, Miao Miao Yang |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
education.field_of_study
Pcr assay Pcr cloning Population Dendrolimus kikuchii nucleopolyhedrovirus Biology Virology Molecular biology law.invention Open reading frame chemistry.chemical_compound chemistry law Insect Science Primer (molecular biology) education Agronomy and Crop Science Polymerase chain reaction DNA |
| Zdroj: | Biocontrol Science and Technology. 22:945-953 |
| ISSN: | 1360-0478 0958-3157 |
| DOI: | 10.1080/09583157.2012.697544 |
| Popis: | A polymerase chain reaction (PCR) assay was developed with a pair of primers, designed based on the unique open reading frame 146 of Dendrolimus kikuchii nucleopolyhedrovirus (DekiNPV). DekiNPV DNA served as a template to amplify 426 bp and 655 bp segments. Results from the sequenced PCR products were consistent with the size of selected primer segments. Two primers were applied to assay DekiNPV DNA and detect intact DekiNPV occlusion bodies (OBs), showing a minimum detection of 0.8 fg/mL DNA and 5 OBs/mL, respectively, indicating the existence of DekiNPV in the infected insects. The assay is characterised with high sensitivity, remarkable specificity and early assay, demonstrating its potential for applications in the studies of DekiNPV host range, substitution of DekiNPV hosts, dynamic DekiNPV changes in D. kikuchii population and routine molecular diagnostic procedures. |
| Databáze: | OpenAIRE |
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