Enhancement of sciatic nerve regeneration after vascular endothelial growth factor (VEGF) gene therapy
| Autor: | B. C. G. Lisboa, Francesco Langone, Fernanda Martins de Almeida, Silvano Lora, Radovan Borojevic, Flávia Frattini, F. R. Pereira Lopes, M.A. Tomaz, Ana Maria Blanco Martinez, Paulo A. Melo, P. K. Matsumoto, Sang Won Han |
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| Rok vydání: | 2011 |
| Předmět: |
Pathology
medicine.medical_specialty Histology biology Angiogenesis Regeneration (biology) Anatomy Spinal cord Pathology and Forensic Medicine Vascular endothelial growth factor chemistry.chemical_compound Gastrocnemius muscle medicine.anatomical_structure Neurology chemistry Physiology (medical) biology.protein medicine Immunohistochemistry Neurology (clinical) Sciatic nerve Neurotrophin |
| Zdroj: | Neuropathology and Applied Neurobiology. 37:600-612 |
| ISSN: | 0305-1846 |
| DOI: | 10.1111/j.1365-2990.2011.01159.x |
| Popis: | F. R. Pereira Lopes, B. C. G. Lisboa, F. Frattini, F. M. Almeida, M. A. Tomaz, P. K. Matsumoto, F. Langone, S. Lora, P. A. Melo, R. Borojevic, S. W. Han and A. M. B. Martinez (2011) Neuropathology and Applied Neurobiology37, 600–612 Enhancement of sciatic nerve regeneration after vascular endothelial growth factor (VEGF) gene therapy Aims: Recent studies have emphasized the beneficial effects of the vascular endothelial growth factor (VEGF) on neurone survival and Schwann cell proliferation. VEGF is a potent angiogenic factor, and angiogenesis has long been recognized as an important and necessary step during tissue repair. Here, we investigated the effects of VEGF on sciatic nerve regeneration. Methods: Using light and electron microscopy, we evaluated sciatic nerve regeneration after transection and VEGF gene therapy. We examined the survival of the neurones in the dorsal root ganglia and in lumbar 4 segment of spinal cord. We also evaluated the functional recovery using the sciatic functional index and gastrocnemius muscle weight. In addition, we evaluated the VEGF expression by immunohistochemistry. Results: Fluorescein isothiocyanate-dextran (FITC-dextran) fluorescence of nerves and muscles revealed intense staining in the VEGF-treated group. Quantitative analysis showed that the numbers of myelinated fibres and blood vessels were significantly higher in VEGF-treated animals. VEGF also increased the survival of neurone cell bodies in dorsal root ganglia and in spinal cord. The sciatic functional index and gastrocnemius muscle weight reached significantly higher values in VEGF-treated animals. Conclusion: We demonstrate a positive relationship between increased vascularization and enhanced nerve regeneration, indicating that VEGF administration can support and enhance the growth of regenerating nerve fibres, probably through a combination of angiogenic, neurotrophic and neuroprotective effects. |
| Databáze: | OpenAIRE |
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