The increased permeability and inflammatory response of porcine intestinal epithelial cells caused by Escherichia coli and Salmonella enterica Typhimurium infections are mitigated by bovine colostrum
| Autor: | Mylene Blais, Michael Bouchard, Vincent Touchette, Claude Asselin, Martin Lessard |
|---|---|
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 202:190.67-190.67 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.202.supp.190.67 |
| Popis: | Gastroenteric infections caused by Escherichia coli and Salmonellaenterica Typhimurium are the source of important losses in porcineindustry. The use of antibiotics in piglets’ food to improve post-weaning performances and prevent enteric infections is raising many concerns, leading the governments to call for finding alternatives that contribute to enhance gut health and resistance to infections. Bovine colostrum is loaded with bioactive pro-immune components that could mediate anti-microbial activities. Therefore, we tested the effect of complete defatted bovine colostrum (BC), as well as bovineserocolostrum and casein fractions (SC and CAS respectively) on porcineintestinal epithelial cell (IPEC J2) in response to the two enteric pathogens. Bothpathogens increased the cell monolayer permeability 4h following the infection, as determined by TEER assays. BC treatment prevented the monolayer disruption caused by both pathogens, while SC only prevented the one caused by Salmonella. After 2h of incubation, the expression of IL8, IL6, TNFA, CCL20, CXCL2 and CXCL10 was increased by bothpathogens, while IL1B, CCL5 and SAA2 were only induced by Salmonella, as measured by Q-PCR. BC and SC reduced the induction of IL8, CCL20 and CXCL2 genes by E. coli(p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|