| Popis: |
Acetonitrile (ACN) is shown to be an effective eluent in combina-tion with methanol (MeOH) or acetone for the rapid separation of polyunsaturated fatty acid methyl esters on a fully silver-loaded ion-exchange (XN1010) column. ACN-containing eluents thus provide on one fully siliver-loaded resin column an attractive alternative separation system to a series of partially silvered resin columns (“PARC” columns), which previously have been used for such separations. Solvent programming from 0 to 15% ACN in MeOH allows the separation of methyl oleate, linoleate, linolenate and arachidonate within 3 hr. Preparative (up to 800 mg) isocratic separations of a number of polyunsaturated fatty acid esters from natural sources (methyl linoleate from safflower esters, for example) were readily achieved. |
Full text from SpringerLink