Smooth muscle responsiveness to IL-13 is sufficient for IL-13 induction of airway hyperresponsiveness (AHR) (39.6)
| Autor: | Fred D. Finkelman, Charles Perkins, Tatyana Orekov, Crystal Potter, George Smulian, Lucy Gildea, Marsha Wills-Karp, Frank Brombacher |
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| Rok vydání: | 2007 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 178:S26-S26 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.178.supp.39.6 |
| Popis: | IL-13 is the primary inducer of AHR in mouse models of asthma and increases contractility of isolated muscle cells in vitro and the contractility of intestinal smooth muscle in worm-infected mice. Consequently, we evaluated whether smooth muscle responsiveness to IL-13 is sufficient for development of AHR in mice inoculated intratracheally with IL-13. The smooth muscle-specific promoter SMP8 was used to generate mice that express IL-4Rα only on smooth muscle cells (SMonly mice, produced by crossing SMP8-IL-4Rα transgenic mice to IL-4Rα-deficient mice). SMonly mice failed to develop goblet cell hyperplasia or BAL eosinophilia in response to intratracheal IL-13 but developed AHR to inhaled methacholine to the same extent as wild-type mice when tested by barometric plethysmography with a Buxco apparatus and significant, albeit reduced AHR to injected acetylcholine when anesthetized, intubated and evaluated by the airway time-pressure index (APTI) technique. Thus, smooth muscle expression of IL-4Rα is sufficient for IL-13 induction of AHR and this is not dependent on goblet cell hyperplasia or pulmonary eosinophilia. Studies designed to determine whether smooth muscle expression of IL-4Rα is necessary for IL-13 induction of AHR are in progress. Supported by the US Dept of Veterans Affairs and NIH. |
| Databáze: | OpenAIRE |
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