Heart and Neural Crest Derivatives Expressed Transcript 2 (HAND2) is Reduced in Women with Breast Cancer. A Case-Control Study
Autor: | Quiti dos Anjos Lopes, Luiza de Azevedo Gross, Ricardo Francalacci Savaris, Ernesto de Paula Guedes Neto, José Luiz Pedrini |
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Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
biology business.industry Case-control study Neural crest medicine.disease 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Breast cancer 030220 oncology & carcinogenesis Cancer research biology.protein General Earth and Planetary Sciences Medicine Immunohistochemistry business HAND2 General Environmental Science |
Zdroj: | Clinical Oncology and Research. :1-6 |
ISSN: | 2613-4942 |
Popis: | Background: The search for new markers for breast cancer (BC) has been sought in order to better understand this type of cancer. The Heart and Neural Crest Derivatives Expressed Transcript 2 (HAND2) has been related to endometrial cancer but there are scant data related to BC. The aim of this study is to compare the immunohistochemical expression of HßAND2 in normal breast tissue vs. BC and to correlate with the estrogen receptor (ERα). Patients and Methods: In this case-control study, 19 formalin–fixed, paraffin-embedded tissues were obtained from pathological archives. Benign (n=9; control) and cancer (n=10) breast tissue were analyzed with immunohistochemistry for HAND2 (Ab60037), at dilution 1:50 at pH 9 and ERα (SP1). ImageJ software with "color deconvolution" was used for analysis of the expression of these proteins. The sample size was calculated (power=95%, α error= 1%) to identify an increase from mean 15 DAB units (control) to 40 DAB units in cancer. Results: HAND2 expression (mean ± SD) was 15.5 ± 6.1 (cancer) versus 44.8 ± 21.1 (control) (P=0.002, Student t-test). Its expression was mainly present in the cytosol of the cells. No correlation was observed between ERα and HAND2 (Pearson r = -0.28 (95%CI=-0.6 to 0.22; P=0.2). Conclusions: The protein expression of HAND2, using Ab60037 antibody, is reduced in breast cancer, compared with normal breast tissue. The expression of HAND2 is not correlated with ERα expression. |
Databáze: | OpenAIRE |
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