| Autor: |
Harry F. Noller, R. B. Siegel, J. Kop, Carl R. Woese, Ramesh Gupta, Linda J. Magrum, A M Kopylov |
| Rok vydání: |
1984 |
| Předmět: |
|
| Zdroj: |
Journal of Biological Chemistry. 259:15287-15293 |
| ISSN: |
0021-9258 |
| Popis: |
A majority (approximately 89%) of the nucleotide sequence of Bacillus brevis 16 S rRNA has been determined by a combination of RNA sequencing methods. Several experimental approaches have been used to probe its structure, including (a) partial RNase digestion of 30 S ribosomal subunits, followed by two-dimensional native/denatured gel electrophoresis, in which base-paired fragments were directly identified; (b) identification of positions susceptible to cleavage by RNase A and RNase T1 in 30 S subunits; (c) sites of attack by cobra venom RNase on naked 16 S rRNA; and (d) nucleotides susceptible to attack by bisulfite in 16 S rRNA. These data are discussed with respect to a secondary structure model for B. brevis 16 S rRNA derived by comparative sequence analysis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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