Critical temperature for production of MAG by esterification of different FA with glycerol using Penicillium camembertii lipase
| Autor: | Praphan Pinsirodom, Akio Sugihara, Takashi Kobayashi, Toshihiro Nagao, Yomi Watanabe, Yuji Shimada |
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| Rok vydání: | 2004 |
| Předmět: |
Quantitative Biology::Biomolecules
Chromatography biology Chemistry General Chemical Engineering Organic Chemistry Rhizopus oryzae Substrate (chemistry) Astrophysics::Cosmology and Extragalactic Astrophysics biology.organism_classification Candida rugosa Catalysis chemistry.chemical_compound nervous system Rhizopus Penicillium biology.protein Glycerol Astrophysics::Solar and Stellar Astrophysics lipids (amino acids peptides and proteins) Astrophysics::Earth and Planetary Astrophysics Lipase Astrophysics::Galaxy Astrophysics |
| Zdroj: | Journal of the American Oil Chemists' Society. 81:543-547 |
| ISSN: | 0003-021X |
| DOI: | 10.1007/s11746-006-0938-z |
| Popis: | Production of MAG by a lipase-catalyzed reaction is known to be effective at low temperature. This phenomenon can be explained by assuming that synthesized MAG are excluded from the reaction system because MAG, which have low m.p., are solidified at low temperatures. Consequently, MAG are efficiently accumulated and do not serve as the precursor of DAG. If this hypothesis is correct, the critical temperature for MAG production, defined as the highest temperature at which DAG synthesis is repressed, should depend on the m.p. of the MAG. Esterification of FFA with glycerol using Candida rugosa, Rhizopus oryzae, and Penicillium camembertii lipases produced MAG efficiently at low temperatures. However, Candida lipase showed very low esterification activity at high temperatures (>20°C), and Rhizopus lipase produced not only MAG but also DAG even at low temperatures. Meanwhile, P. camembertii lipase catalyzed synthesis of MAG only from FFA and glycerol at low temperatures, although the enzyme catalyzed synthesis of DAG from MAG in addition to synthesis of MAG at high temperatures. We thus studied the effect of temperature on esterification of C10−C18 FFA with glycerol using Penicillium lipase as a catalyst and determined the critical temperatures for production of MAG. The critical temperature for production of each MAG showed a linear correlation with m.p. of the MAG, which supported the hypothesis. In addition, because the m.p. of MAG are estimated from that of the constituent FA, the optimal temperature for production of MAG can be predicted from the m.p. of the FFA used as a substrate. |
| Databáze: | OpenAIRE |
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