The Gene Expression of Dynorphin, Enkephalin, c-Fos in the Neuropathic Rat
| Autor: | Sug Hwa Yoon, Hye Ja Kim, Won Jae Hwang, Byung Doo Hwang, Sae Jin Choi, Won Hyung Lee, Soo Chang Son, Yong Sup Shin, Kyu Lim, Neung Hee Han |
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| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
biology Enkephalin business.industry musculoskeletal neural and ocular physiology Dynorphin c-Fos Anesthesiology and Pain Medicine Endocrinology Allodynia Internal medicine Gene expression Neuropathic pain medicine biology.protein Northern blot medicine.symptom business Guanethidine medicine.drug |
| Zdroj: | Korean Journal of Anesthesiology. 31:293 |
| ISSN: | 0302-5780 |
| DOI: | 10.4097/kjae.1996.31.3.293 |
| Popis: | Background: We studied the time course of gene expression of dynorphin, enkephalin, c-fos, and the changes of allodynia, and the effect of chemical sympathectomy on the gene expression and allodynia in neuropathic rat. Methods: In two groups of rat (Sprague-Dawley), the left L5 and L6 spinal nerves were tight ligated. In gene expression group (N=25), behavioral tests for mechanical allodynia and cold allodynia were perfomed for the next two weeks. After the test of allodynia, the expression of dynorphin, enkephalin, c-fos were assessed by Northern blot hybridization. In chemical sympathectomy group (N=16), after chemical sympathectomy (guanethidine 70 mg/kg intraperitoneally, from postoperative 7 days to 9 days), the changes of allodynia and the gene expression of enkephalin, c-fos were tested. Results: Mechanical allodynia and cold allodynia was developed on the postoperative 3, 5, 7, 14 days. Preprodynorphin mRNA expression was reached peak level at the postoperative 8 hrs, sustained increase by the postoperative 3 days, but preproenkephalin mRNA expression increased slightly after operation. c-Fos mRNA expression was increased immediately at the postoperative 30 min, 1 hr, returned to normal level thereafter, and increased again on the postoperative 3, 5, 7 days that neuropathic pain was developed. Mechanical allodynia and cold allodynia were decreased by chemical sympathectomy. The increased c-fos mRNA expression and pain at postoperative 7 days was reduced by chemical sympathectomy. Conclusion: These results suggest that the transient gene expression of dynorphin and c-fos after tight ligation of L5 and L6 spinal nerves induces the development neuropathic pain, and late c-fos expression is related to neuropathic pain. (Korean J Anesthesiol 1996; 31: 293-303) |
| Databáze: | OpenAIRE |
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