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Summary A dextran-polyethylene glycol two-phase system was used to separate an endomembrane fraction involved in sesquiterpene hydrocarbon biosynthesis, from organelles elaborating monoterpene hydrocarbons in the exocarp of CitrofortunelLa mitis (calamondin) fruits. Endomembranes showed a high affinity for the polyethylene glycol-rich upper phase whereas organelles partitioned in the dextran-rich lower phase. The membranes obtained in the polyethylene glycol phase were purified on a sucrose density gradient. A fraction enriched in endoplasmic reticulum, characterized by enzyme markers, was isolated. In the presence of [1- 14 C] isopentenyl pyrophosphate, this fraction was able to synthesize ,β-selinene and germacrene D, the main CIS hydrocarbons present in the oil of calamondin fruits. |
