A fully automated robotic system for three-dimensional cell rotation
| Autor: | Peng Cheang Wong, Wei Tech Ang, Win Tun Latt, Steven Yih Min Tan, Ramadass Muruganandam, Chen Feng, Joyce Mathew, Zenan Wang |
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| Rok vydání: | 2016 |
| Předmět: |
0209 industrial biotechnology
In vitro fertilisation Computer science Orientation (computer vision) medicine.medical_treatment 0206 medical engineering Cell Embryo 02 engineering and technology Blastomere Preimplantation genetic diagnosis 020601 biomedical engineering Intracytoplasmic sperm injection 020901 industrial engineering & automation medicine.anatomical_structure medicine Simulation Biomedical engineering |
| Zdroj: | ICRA |
| Popis: | Injection and extraction of materials (e.g. protein, sperms, DNA, and blastomeres) into and from cells are essential operation for In Vitro Fertilisation (IVF), intracytoplasmic sperm injection (ICSI) and preimplantation genetic diagnosis (PGD). In order to perform the injection and extraction without damage to the cell, the cell needs to be at an optimal orientation. In this paper, a fully automated microrobotic system for cell orientation is presented. The proposed system overcomes several inherent problems in manual cell manipulation, including low efficiency, inconsistent output and poor success rate. The system can rotate a single fish embryo to a desired orientation by employing motion control, fluidic flow control, and computer vision. It is evaluated by using 60 Zebrafish embryos. Experimental results show that the system is capable of performing fast embryo orientation at a speed of 13 s/cell with a high success rate of 97.5% and a high in-plane rotation precision of 0.5° |
| Databáze: | OpenAIRE |
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