The L4F3 antigen is expressed by unipotent and multipotent colony- forming cells but not by their precursors
| Autor: | RG Andrews, M Takahashi, GM Segal, JS Powell, ID Bernstein, JW Singer |
|---|---|
| Rok vydání: | 1986 |
| Předmět: | |
| Zdroj: | Blood. 68:1030-1035 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood.v68.5.1030.bloodjournal6851030 |
| Popis: | Antibody L4F3 is a murine monoclonal antibody that recognizes an antigen expressed on in vitro colony-forming cells, including virtually all CFU-GM, CFU-Meg, BFU-E, and CFU-Mix. In the present study we examined whether cells that do not express the L4F3 antigen include precursors of hematopoietic colony-forming cells. Colony-forming cells were depleted from marrow by treatment with L4F3 and complement. The remaining cells generated CFU-GM, BFU-E, and CFU-Mix when cultured in the presence of irradiated adherent cell layers from long-term marrow cultures. Marrow cells not expressing the L4F3 antigen, which were separated by cell-sorting techniques, were depleted of colony-forming cells but nevertheless generated CFU-GM when cultured over irradiated adherent cell layers. These data suggest that there are marrow precursors that do not express the L4F3 antigen and that give rise to colony-forming cells of multiple types. Negative selection techniques should allow the enrichment of these precursors of colony-forming cells, thereby enabling direct studies of these immature stem cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|