High anti-TNP plaque-forming cell potential of residual mIg+ cells in a T cell population
| Autor: | J J Mond, M G Mage, T L Rothstein, D E Mosier, H Herrod, R Asofsky, W E Paul |
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| Rok vydání: | 1980 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 125:1526-1529 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.125.4.1526 |
| Popis: | In the course of experiments designed to study the immune response of purified populations of B lymphocytes to thymus-independent (TI) antigens, a variety of cell purification procedures were followed. In using anti-immunoglobulin-coated dishes to separate lymphocytes bearing membrane immunoglobulin (mIg) from mIg- lymphocytes, it was found that the nonadherent fraction, which was predominantly mIg-, complement receptor negative, and nonresponsive to the B cell mitogen lipopolysaccharide, gave very substantial anti-TNP plaque-forming cell responses to 2 TI antigens. These responses could be inhibited by incubation of such cells in the presence of anti-mu and thus appeared to be attributable to mIg+ cells. The evidence suggests the existence of a population of B lymphocytes that constitute a minor component of mIg+ cells having a high potential to make in vitro antibody responses. Users of techniques that utilize anti-Ig as a tool for separating B and T lymphocytes should carefully assess the extent to which residual B lymphocytes in the mIg- population contribute to antibody responses being studied. |
| Databáze: | OpenAIRE |
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