The biotransformation of chrysene to trans-1,2-dihydroxy-1,2-dihydrochrysene by filamentous fungi
| Autor: | Eberhard Kiehlmann, Linda J. Pinto, Margo M. Moore |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Chrysene
biology Bioconversion Stereochemistry Immunology General Medicine Fungi imperfecti biology.organism_classification Applied Microbiology and Biotechnology Microbiology chemistry.chemical_compound Bioremediation chemistry Biotransformation Penicillium Genetics Pyrene Organic chemistry Molecular Biology Syncephalastrum racemosum |
| Zdroj: | Canadian Journal of Microbiology. 42:604-608 |
| ISSN: | 1480-3275 0008-4166 |
| DOI: | 10.1139/m96-081 |
| Popis: | The purpose of this study was to assess the ability of filamentous fungi isolated from petroleum-contaminated soils to oxidize chrysene. Only 4 of the 17 isolates known to oxidize pyrene and benzo[a]pyrene were found to produce polar products when incubated in the presence of chrysene and Tween 80: Penicillium janthinellum, Syncephalastrum racemosum, and 2 Penicillium spp. Trows-1,2-dihydroxy-1,2-dihydrochrysene was identified by 1H-NMR as one of three fungal metabolites. The extent of bioconversion to diol was approximately 3% of chrysene in 6 days. Experiments to increase chrysene oxidation with other polycyclic aromatic hydrocarbons were not successful. To our knowledge, this is the first identification of a chrysene metabolite from any microorganism and the first report of fungal oxidation of chrysene.Key words: chrysene, polycyclic aromatic hydrocarbons, filamentous fungi, bioremediation, cytochrome P450. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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