Detection of Post-translationally Modified p53 by Western Blotting
| Autor: | David W. Meek, Anna Estevan Barber |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
biology medicine.diagnostic_test DNA damage Cellular differentiation Methylation Cell biology Blot 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Ubiquitin Western blot Acetylation 030220 oncology & carcinogenesis biology.protein medicine Phosphorylation |
| Zdroj: | Cell Cycle Checkpoints ISBN: 9781071612163 |
| DOI: | 10.1007/978-1-0716-1217-0_2 |
| Popis: | The p53 tumor suppressor has a central role in many key cellular processes including the DNA damage response, aging, stem cell differentiation, and fertility. p53 undergoes extensive regulatory post-translational modification through events such as phosphorylation, acetylation, methylation, and ubiquitylation. Here, we describe western blotting-based methodology for the detection and relative quantification of individual phosphorylation events in p53. While we focus on well-established N-terminal modifications for the purpose of illustration, this approach can be used to investigate other post-translational modifications of the protein, drawing upon a broad range of commercially available modification-specific antibodies. |
| Databáze: | OpenAIRE |
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