P.1.001 Insertion/deletion polymorphism of the angiotensin I-converting enzyme gene in migraine patients

Autor: G. Schiavo, A. Bisogno, A. Bianchi, A. Agresta, F. Infante, Anna Capasso, E. Lamaida, G. Volpe, Vincenzo Pizza
Rok vydání: 2007
Předmět:
Zdroj: European Neuropsychopharmacology. 17:S147
ISSN: 0924-977X
2005-2006
DOI: 10.1016/s0924-977x(07)70130-x
Popis: Several authors reported an association between the Angiotensin Converting Enzyme (ACE)-D allele and coronary artery disease. The mechanism on the basis of this association is unclear. Recently, it has been suggested that the ACE-DD polymorphism may play an important role in the determinism and in the frequency of migraine attacks. Theredore, the aim of our study was to determine the incidence of ACE polymorphism in a consecutive series of migrainous patients (ICHDII-2004 diagnostic criteria) and of patients affected by myocardial infarction. We studied a series of 51 migrainous patients aged 38.6 years +/- 18.8 (8 MWA and 43 MwA, ICHDII-2004 criteria) come at our observation in the period 2005-2006. The control group was composed by 58 patients affected by Acute Myocardial Infarction (AMI) admitted to the ICCU (Intensive Coronary Care Unit) of S.Luca Hospital in Vallo della Lucania in the same period. Exclusion criteria from the study were: positive anamnesis for analgesic abuse, presence of serious diseases, need to take drugs for other pathologies. The analyse was based on Polymerase Chain Reaction (PCR) and on reverse-hybridization and included 3 steps: 1 DNA isolation from non coagulated blood, 2 PCR amplification using biotinylated primers, 3 hybridization of amplification products to a test strip containing allele-specific oligonucleotide probes immobilized as an array of parallel lines. Bound biotinylated sequences were detected using streptavidin-alkaline phosphatase and an appropriate color substrate. 16 (42%) migrainous patients and 32 (56%) cardiopathy patients had an ID genotype; 19 (50%) migraineurs and 20 (34%) cardiopaths had a DD genotype. The results of our study confirm the association between migraine and ACE I/D genetic polymorphism. These data are confirmed in the sample of patients affected by myocardial infarction. This gives evidence of a strong relationship between migraine and major vascular diseases and let us hypothesize an important role of ACE system in the pathogenetic model of migraine for its capability to interfere with the endothelial regulation tone. Once an effective role in the genesis of migraine and in the increased risk of migrainous patients to evolve into an ischemic pathology has been obviously assigned to this genetic mutation, future researches must aim through wider and more controlled casistics also to clarify the role that drugs acting on these systems may have on the resolution of these diseases.
Databáze: OpenAIRE
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