New insights on the spectrophotometric determination of melatonin pKa values and melatonin-βCD inclusion complex formation constant
| Autor: | Manuel Palomar-Pardavé, S. Corona-Avendaño, Ma. Teresa Ramírez-Silva, M. Romero-Romo, A. Zafra-Roldán, R. Montes-Sánchez |
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| Rok vydání: | 2018 |
| Předmět: |
Molar
Chromatography medicine.diagnostic_test Complex formation chemistry.chemical_element 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Oxygen Atomic and Molecular Physics and Optics 0104 chemical sciences Analytical Chemistry Melatonin chemistry Stability constants of complexes Spectrophotometry medicine Physical chemistry A value 0210 nano-technology Constant (mathematics) Instrumentation Spectroscopy medicine.drug |
| Zdroj: | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 190:442-449 |
| ISSN: | 1386-1425 |
| Popis: | Using UV-Vis spectrophotometry a stability study of melatonin at different pH values was done in aqueous media, finding that at acidic pH melatonin is unstable when interacting with the environment, however it becomes stable protecting it from light and oxygen. From the UV-Vis spectra and SQUAD software, melatonin pKa values, in a completely protected aqueous medium, were estimated as 5.777±0.011 and 10.201±0.024. Using the same techniques, the melatonin and β-cyclodextrin inclusion complex formation constants were assessed at pH3, 7 and 11.5, giving the values of log β=(3.07±0.06), (2.94±0.01) and (3.07±0.06) M-1, respectively. From the global acidity formation constants and the complexes' formation constants, the molar fractions were determined for each species of MT and MT-βCD, to build the molar fraction-[βCD]-pH 3D diagram and the molar fraction-pH 2D diagrams, where it was possible to observe the predominance of the MT species with and without βCD. A voltammetric study at pH3, allowed obtaining a value of log β=(3.15±0.01) M-1, which corroborates that obtained through UV-Vis spectrophotometry, supporting strongly the rationale behind using simple, straightforward techniques. |
| Databáze: | OpenAIRE |
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