Development of technology for purification of plasmid DNAs for pharmaceutical purposes

Autor:	N. I. Akulova, Yu. V. Telegina, G. M. Levagina, Ya. S. Gogina, V. F. Podgornyi, E. A. Volosnikova, V. K. Mikhailova, Lebedev Lr
Rok vydání:	2010
Předmět:	Plasmid preparation chemistry.chemical_classification Biology Applied Microbiology and Biotechnology Biochemistry Molecular biology DNA vaccination chemistry.chemical_compound Plasmid chemistry Plasmid dna Nucleotide DNA
Zdroj:	Applied Biochemistry and Microbiology. 46:865-869
ISSN:	1608-3024 0003-6838
DOI:	10.1134/s0003683810090085
Popis:	Optimization of the stage of fractioning of nucleotide material has been performed, and a technology for purification and obtaining of plasmid DNAs for pharmaceutical purposes has been developed. Plasmid DNAs can be used for construction of DNA vaccines to urgent viral infections. Conditions for extraction and preliminary purification of DNA by salt and alcohol precipitations were determined. An effective gel-filtration procedure of separation of plasmid DNA from contaminants was suggested.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::b491925f0295432c63aac0ca50494c3c https://doi.org/10.1134/s0003683810090085 Zobrazit plný text záznamu Plný text ve formátu PDF