Improved Mitochondrial and Methylglyoxal-Related Metabolisms Support Hyperproliferation Induced by 50 Hz Magnetic Field in Neuroblastoma Cells
| Autor: | Stefano Falone, Marta Grannonico, Marisa Cacchio, Fernanda Amicarelli, Silvano Santini, Patrizia Cesare, Valeria Cordone, Silvia Di Loreto |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Genetics Physiology Cell growth Clinical Biochemistry Cell Methylglyoxal Cellular homeostasis Cell Biology Mitochondrion Biology medicine.disease_cause Phenotype Cell biology 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure chemistry Cell culture 030220 oncology & carcinogenesis medicine Carcinogenesis |
| Zdroj: | Journal of Cellular Physiology. 231:2014-2025 |
| ISSN: | 0021-9541 |
| DOI: | 10.1002/jcp.25310 |
| Popis: | Extremely low frequency magnetic fields (ELF-MF) are common environmental agents that are suspected to promote later stages of tumorigenesis, especially in brain-derived malignancies. Even though ELF magnetic fields have been previously linked to increased proliferation in neuroblastoma cells, no previous work has studied whether ELF-MF exposure may change key biomolecular features, such as anti-glycative defence and energy re-programming, both of which are currently considered as crucial factors involved in the phenotype and progression of many malignancies. Our study investigated whether the hyperproliferation that is induced in SH-SY5Y human neuroblastoma cells by a 50 Hz, 1 mT ELF magnetic field is supported by an improved defense towards methylglyoxal (MG), which is an endogenous cancer-static and glycating α-oxoaldehyde, and by rewiring of energy metabolism. Our findings show that not only the ELF magnetic field interfered with the biology of neuron-derived malignant cells, by de-differentiating further the cellular phenotype and by increasing the proliferative activity, but also triggered cytoprotective mechanisms through the enhancement of the defense against MG, along with a more efficient management of metabolic energy, presumably to support the rapid cell outgrowth. Intriguingly, we also revealed that the MF-induced bioeffects took place after an initial imbalance of the cellular homeostasis, which most likely created a transient unstable milieu. The biochemical pathways and molecular targets revealed in this research could be exploited for future approaches aimed at limiting or suppressing the deleterious effects of ELF magnetic fields. J. Cell. Physiol. 231: 2014-2025, 2016. © 2016 Wiley Periodicals, Inc. |
| Databáze: | OpenAIRE |
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